¹Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, ON M5G 1L6
²Department of Biochemistry and Biophysics, Box 712, University of Rochester School of Medicine, Rochester, NY 14642
³Department of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto, ON M5S 1A8

Abstract:

Using a microarray that tiles all known yeast noncoding RNAs, we compared RNA from wildtype cells to RNA from mutants encoding known and putative RNA modifying enzymes. We show that at least five types of RNA modification (dihydrouridine, m1G, m22G, m1A, m62A) catalyzed by ten different enzymes (Trm1p, Trm5, Trm10p, Dus1p-Dus4p, Dim1p, Gcd10p and Gcd14p) can be detected by virtue of differential hybridization to oligonucleotides on the array that are complementary to the modified sites. Using this approach, we identified a previously undetected modification in GlnCTG tRNA.

Supplementary Data:

Master File

The title of each data file contains the mutated gene, the type of mutation and the concentration of formamide in which the hybridization was performed. These files contain data that has been spatially detrended and normalized (see Materials and Methods).