LOCUS       dna                      5478 bp                                   
FEATURES             Location/Qualifiers
     misc_binding    5..10
                     /dbxref="REBASE:BglII"
     Promoter        25..43
                     /gene="T7 prom"
     misc_binding    63..68
                     /dbxref="REBASE:XbaI"
     Tag             105..768
                     /gene="GST tag"
     ORF             105..989
                     /sequence="ORF_1 rf(3)"
     misc_binding    539..544
                     /dbxref="REBASE:BclI"
     misc_binding    796..803
                     /dbxref="REBASE:NotI"
     misc_binding    824..829
                     /dbxref="REBASE:KpnI"
     misc_binding    858..863
                     /dbxref="REBASE:ApaI"
     misc_binding    877..882
                     /dbxref="REBASE:NcoI"
     misc_binding    897..902
                     /dbxref="REBASE:StuI"
     misc_binding    960..965
                     /dbxref="REBASE:SacI"
     misc_binding    975..980
                     /dbxref="REBASE:BamHI"
     Terminator      983..1111
                     /gene="T7 term"
     misc_binding    1462..1467
                     /dbxref="REBASE:ClaI"
     misc_binding    1487..1492
                     /dbxref="REBASE:EcoRI"
     misc_binding    1487..1492
                     /dbxref="REBASE:ApoI"
     Promoter        1628..1656
                     /gene="amp prom"
     Marker          1698..2558
                     /gene="amp marker"
     ORF             1698..2558
                     /sequence="ORF_2 rf(3)"
     misc_binding    1941..1946
                     /dbxref="REBASE:HincII"
     Rep_Origin      2713..3332
                     /gene="pBR322 origin"
     misc_binding    2959..2967
                     /dbxref="REBASE:AlwNI"
     misc_binding    3604..3609
                     /dbxref="REBASE:AccI"
     Other Gene      3747..3938
                     /gene="ROP other"
     misc_binding    3784..3789
                     /dbxref="REBASE:PvuII"
     ORF             4577..5194
                     /sequence="ORF_3 rf(6)"
     misc_binding    4876..4881
                     /dbxref="REBASE:NruI"
     misc_binding    5290..5295
                     /dbxref="REBASE:SphI"
BASE COUNT    1347 a   1371 c   1471 g   1289 t    0 others
ORIGIN
        1 atcgagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc     
       61 cctctagaaa taattttgtt taactttaag aaggagatat acatatgtcc cctatactag     
      121 gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag     
      181 aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt     
      241 ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa     
      301 cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc     
      361 caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg     
      421 tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc     
      481 tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg     
      541 atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg     
      601 acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta     
      661 tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct     
      721 ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtccat     
      781 gcgagctgaa tccatgcggc cgcaagcttg acgtccatat gctggtaccc cgtggaagtc     
      841 tggaagttct cttccagggg cccgaaggag acgccaccat ggggcatatg gtgcacaggc     
      901 ctttccaatc cgcggccagt taccccaacc ggcccagcaa gacacccccc cgagctgcag     
      961 agctcgctac gtttggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct     
     1021 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt     
     1081 tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat     
     1141 cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg     
     1201 gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag     
     1261 cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg     
     1321 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac     
     1381 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga     
     1441 taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac     
     1501 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt     
     1561 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     
     1621 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     
     1681 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg     
     1741 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg     
     1801 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc     
     1861 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat     
     1921 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact     
     1981 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca     
     2041 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact     
     2101 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg     
     2161 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg     
     2221 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg     
     2281 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg     
     2341 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag     
     2401 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc     
     2461 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga     
     2521 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat     
     2581 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     
     2641 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     
     2701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     
     2761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     
     2821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc     
     2881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     
     2941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     
     3001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     
     3061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     
     3121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     
     3181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     
     3241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     
     3301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct     
     3361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta     
     3421 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag     
     3481 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta     
     3541 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag     
     3601 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca     
     3661 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct     
     3721 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg     
     3781 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt     
     3841 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag     
     3901 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga     
     3961 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt     
     4021 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg     
     4081 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat     
     4141 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg     
     4201 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat     
     4261 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc     
     4321 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac     
     4381 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg     
     4441 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc     
     4501 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg     
     4561 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg     
     4621 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg     
     4681 ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa     
     4741 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca     
     4801 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg     
     4861 ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc     
     4921 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt     
     4981 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc     
     5041 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt     
     5101 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga     
     5161 aggagctgac tgggttgaag gctctcaagg gcatcggtcg atcgacgctc tcccttatgc     
     5221 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca     
     5281 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc     
     5341 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg     
     5401 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg     
     5461 atgcgtccgg cgtagagg                                                   
//
