Zfp206 regulates ES cell gene expression and differentiation
Wen Zhang1, Emily Walker2, Owen Tamplin1, Janet Rossant1, 3, William L. Stanford2
and Timothy R. Hughes1, 4*
1Department of Molecular and Medical Genetics,
2Institute for Biomaterials and Biomedical Engineering,
3The Hospital for Sick Children,
4Banting and Best Department of Medical Research,
*To whom correspondence should be addressed:
416-946-8260
FAX: 416-978-8528
Summary:
Understanding transcriptional regulation in early developmental stages is fundamental to understanding mammalian development and Embryonic Stem (ES) cell properties. Expression surveys suggest that the putative SCAN-Zinc finger transcription factor Zfp206 is expressed specifically in ES cells (1,2). Here, we confirm this observation, and we show that ZFP206 expression decreases rapidly upon differentiation of cultured mouse ES cells, and during development of mouse embryos. We find that there are at least six isoforms of the ZFP206 transcript, the longest being predominant. Overexpression and depletion experiments show that Zfp206 promotes formation of undifferentiated ES cell clones, and positively regulates abundance of a very small set of transcripts whose expression is also specific to ES cells and the two- to four-cell stages of preimplantation embryos. This set includes members of the Zcsan4, Thoc4, Tcstv1, and eIF-1A gene families, none of which have been functionally characterized in vivo but whose members include apparent transcription factors, RNA-binding proteins, and translation factors. Together, these data indicate that Zfp206 is a regulator of ES cell differentiation that controls a set of genes expressed very early in development, most of which themselves appear to be regulators.
Supplementary Data:
ZFP206 Microarray Data 1 (LogIntensity)| Last Updated on August 22, 2006 Site created by Wen Zhang |